Functionalized nanopipettes: toward label-free, single cell biosensors

Nanopipette technology has been proven to be a label-free biosensor capable of identifying DNA and proteins. The nanopipette can include specific recognition elements for analyte discrimination based on size, shape, and charge density. The fully electrical read-out and the ease and low-cost fabrication are unique features that give this technology an enormous potential. Unlike other biosensing platforms, nanopipettes can be precisely manipulated with submicron accuracy and used to study single cell dynamics. This review is focused on creative applications of nanopipette technology for biosensing. We highlight the potential of this technology with a particular attention to integration of this biosensor with single cell manipulation platforms

Nanopore Device for Reversible Ion and Molecule Sensing or Migration

Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore

Morphology and Light‐Dependent Spatial Distribution of Spin Defects in Carbon Nitride

Carbon nitride (CN) is a heterogeneous photocatalyst that combines good structural properties and a broad scope. The photocatalytic efficiency of CN is associated with the presence of defective and radical species. An accurate description of defective states—both at a local and extended level—is key to develop a thorough mechanistic understanding of the photophysics of CN. In turn, this will maximise the generation and usage of photogenerated charge carriers and minimise wasteful charge recombination. Here the influence of morphology and light-excitation on the number and chemical nature of radical defects is assessed. By exploiting the magnetic dipole-dipole coupling, the spatial distribution of native radicals in CN is derived with high precision. From the analysis an average distance in the range 1.99–2.34 nm is determined, which corresponds to pairs of radicals located approximately four tri-s-triazine units apart

Dataset for Automated Purification of DNA Origami with SPRI Beads

The data set contains relevant information for the associated manuscript "Automated Purification of DNA Origami with SPRI Beads". It contains the Supporting Information 2, the origami design file and AFM images used in the publication

Data for "Mechanistic Study of the Conductance and Enhanced Single-Molecule Detection in a Polymer Electrolyte Nanopore"

The data set contains all relevant ionic current traces and voltammetric data recorded using pClamp system for the associated paper entitled: 'Mechanistic Study of the Conductance and Enhanced Single-Molecule Detection in a Polymer Electrolyte Nanopore'. The data is in the native pClamp format of abf files. Analysis routine can be performed with script that relies on abf files. The data set is aim to allow users to re-analysis the data and to reproduce the observations made in the associated publication